Certified Surgical Technologist (CST) Practice Exam

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At what degrees Celsius is the steam sterilization biological indicator incubated?

  1. 43-48

  2. 55-60

  3. 61-66

  4. 49-54

The correct answer is: 55-60

The steam sterilization biological indicator is incubated at a temperature range of 55 to 60 degrees Celsius. This temperature range is optimal for the growth of bacterial spores used in biological indicators, allowing for the most accurate assessment of the sterilization process. Incubating at this temperature ensures that if the sterilization process was ineffective, bacterial growth would indicate a failure, enabling surgical teams to take necessary corrective actions. This is a crucial aspect of quality control in sterilization procedures, ensuring that all surgical instruments and materials are adequately sterilized before use.